NOVA2 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5998
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5998-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the NOVA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human appendicitis tissue with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 7
IHC staining of FFPE mouse brain with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE mouse brain with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE rat brain with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
Western blot testing of human 1) U-2 OS, 2) HL60, 3) ThP-1, 4) rat brain, 5) rat spleen, 6) rat lung and 7) mouse brain lysate with NOVA2 antibody. Expected molecular weight: 70-80 kDa with a 50-55 kDa band also sometimes seen (Ref 1).
6 / 7
Flow cytometry testing of human A549 cells with NOVA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOVA2 antibody.
7 / 7
Flow cytometry testing of mouse HEPA1-6 cells with NOVA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOVA2 antibody.
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IHC staining of FFPE human appendicitis tissue with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with NOVA2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) U-2 OS, 2) HL60, 3) ThP-1, 4) rat brain, 5) rat spleen, 6) rat lung and 7) mouse brain lysate with NOVA2 antibody. Expected molecular weight: 70-80 kDa with a 50-55 kDa band also sometimes seen (Ref 1).
Flow cytometry testing of human A549 cells with NOVA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOVA2 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with NOVA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NOVA2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NOVA2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
NOVA alternative splicing regulator 2 is a protein that in humans is encoded by the NOVA2 gene. It is mapped to 19q13.32. NOVA2 may regulate RNA splicing or metabolism in a specific subset of developing neurons. It binds single strand RNA.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-Q205) was used as the immunogen for the NOVA2 antibody.
Limitation:
This NOVA2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UNW9