PCBP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6205
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6205-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PCBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human tonsil with PCBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer with PCBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer with PCBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate cancer with PCBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of 1) human HeLa, 2) human HepG2 and 3) mouse NIH 3T3 lysate with PCBP1 antibody. Predicted molecular weight ~38 kDa.
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Flow cytometry testing of human Caco-2 cells with PCBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCBP1 antibody.
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Flow cytometry testing of mouse RAW264.7 cells with PCBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCBP1 antibody.
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Flow cytometry testing of rat RH35 cells with PCBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCBP1 antibody.
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IHC staining of FFPE human tonsil with PCBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with PCBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with PCBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer with PCBP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human HepG2 and 3) mouse NIH 3T3 lysate with PCBP1 antibody. Predicted molecular weight ~38 kDa.
Flow cytometry testing of human Caco-2 cells with PCBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCBP1 antibody.
Flow cytometry testing of mouse RAW264.7 cells with PCBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCBP1 antibody.
Flow cytometry testing of rat RH35 cells with PCBP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCBP1 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PCBP1 antibody should be determined by the researcher.
Description:
Poly(rC)-binding protein 1 is a protein that in humans is encoded by the PCBP1 gene. This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids P152-K268) was used as the immunogen for the PCBP1 antibody.
Limitation:
This PCBP1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15365