PHB Antibody / Prohibitin

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NSJ Bioreagents
Product Code: NSJ-RQ6392
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6392-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PHB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 11
IHC staining of FFPE human ovarian cancer with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 11
IHC staining of FFPE human breast cancer with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC staining of FFPE human liver cancer with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human lung cancer with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human adrenocortical adenoma tissue with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human rectal cancer tissue with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE mouse brain tissue with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE rat brain tissue with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) K562, 5) HT1080, 6) SW620, 7) U-87 MG, 8) A549, 9) rat brain, 10) rat heart, 11) rat liver, 12) rat PC-12, 13) mouse brain, 14) mouse heart, 15) mouse liver and 16) mouse NIH 3T3 cell lysate with PHB antibody. Predicted molecular weight ~29 kDa.
10 / 11
Flow cytometry testing of human HL60 cells with PHB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PHB antibody.
11 / 11
Flow cytometry testing of rat RH35 cells with PHB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PHB antibody.
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IHC staining of FFPE human ovarian cancer with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adrenocortical adenoma tissue with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with PHB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) Jurkat, 3) HEK293, 4) K562, 5) HT1080, 6) SW620, 7) U-87 MG, 8) A549, 9) rat brain, 10) rat heart, 11) rat liver, 12) rat PC-12, 13) mouse brain, 14) mouse heart, 15) mouse liver and 16) mouse NIH 3T3 cell lysate with PHB antibody. Predicted molecular weight ~29 kDa.
Flow cytometry testing of human HL60 cells with PHB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PHB antibody.
Flow cytometry testing of rat RH35 cells with PHB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PHB antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PHB antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
PHB (Prohibitin), also known as PHB1, is a protein that in humans is encoded by the PHB gene. White et al.(1991) mapped the PHB gene to chromosome 17 by analysis of human-mouse somatic cell hybrid cell lines using a genomic fragment of human prohibitin DNA isolated from a library using the rat prohibitin cDNA clone. By in situ hybridization, they localized the gene to 17q21. Sato et al.(1992) isolated the human homolog of the rat prohibitin gene and mapped it to 17q12-q21 by in situ hybridization. Proliferation of tumor cells depends on new blood vessel formation(angiogenesis) that accompanies malignant progression. Anticancer therapies using angiogenesis inhibitors or cytotoxic agents targeted to the vasculature of tumors have been evaluated in clinical trials. Although white fat is a nonmalignant tissue, it has the capability to quickly proliferate and expand. Furthermore, it is highly vascularized. Rupnick et al.(2002) showed that nonspecific angiogenesis inhibitors can prevent the development of obesity of mice.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-I257) was used as the immunogen for the PHB antibody.
Limitation:
This PHB antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35232