PI3K gamma Antibody / PIK3CG

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NSJ Bioreagents
Product Code: NSJ-RQ5751
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5751-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PI3K gamma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human breast cancer with PI3K gamma antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human 1) K562, 2) Raji, 3) Jurkat, 4) HepG2, 5) rat RH-35, 6) rat PC-12, 7) NIH 3T3 and 8) RAW264.7 lysate with PI3K gamma antibody. Expected molecular weight: 110-126 kDa.
3 / 3
Flow cytometry testing of human Raji cells with PI3K gamma antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PI3K gamma antibody.
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IHC staining of FFPE human breast cancer with PI3K gamma antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562, 2) Raji, 3) Jurkat, 4) HepG2, 5) rat RH-35, 6) rat PC-12, 7) NIH 3T3 and 8) RAW264.7 lysate with PI3K gamma antibody. Expected molecular weight: 110-126 kDa.
Flow cytometry testing of human Raji cells with PI3K gamma antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PI3K gamma antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the PI3K gamma antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform is an enzyme that in humans is encoded by the PIK3CG gene. Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids K52-D308) was used as the immunogen for the PI3K gamma antibody.
Limitation:
This PI3K gamma antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P48736