Semaphorin 6A Antibody / SEMA6A

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NSJ Bioreagents
Product Code: NSJ-RQ5921
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5921-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Semaphorin 6A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human rectal cancer with Semaphorin 6A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of human 1) HEK293, 2) HeLa, 3) Raji, 4) U-2 OS, 5) Jurkat, 6) Caco-2, 7) rat brain, 8) rat PC-12, 9) mouse brain and 10) mouse RAW264.7 with Semaphorin 6A antibody. Predicted molecular weight ~114 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with Semaphorin 6A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEMA6A antibody.
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IHC staining of FFPE human rectal cancer with Semaphorin 6A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HEK293, 2) HeLa, 3) Raji, 4) U-2 OS, 5) Jurkat, 6) Caco-2, 7) rat brain, 8) rat PC-12, 9) mouse brain and 10) mouse RAW264.7 with Semaphorin 6A antibody. Predicted molecular weight ~114 kDa.
Flow cytometry testing of human HepG2 cells with Semaphorin 6A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEMA6A antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Semaphorin 6A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
SEMA6A belongs to a subfamily characterized by an extracellular semaphorin domain, a transmembrane domain, and a long cytoplasmic tail. Members of this class can repel sympathetic and dorsal root ganglion axons in vitro, consistent with a traditional role as guidance signals. However, the length of the cytoplasmic tail, which includes an EVL-binding site in SEMA6A and an Src-binding site in SEMA6B, suggests that these semaphorins may also function as receptors. SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection. SEMA6A directly links the Ena/VASP and the semaphorin protein families since the SEMA6A protein is capable of selective binding to the protein EVL (Ena/VASP-like protein).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids H31-N306) was used as the immunogen for the Semaphorin 6A antibody.
Limitation:
This Semaphorin 6A antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9H2E6