VAMP4 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6405
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6405-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the VAMP4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human breast cancer with VAMP4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE mouse brain tissue with VAMP4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat brain tissue with VAMP4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) U-87 MG, 2) T-47D, 3) K562, 4) PC-3, 5) ThP-1, 6) HEK293, 7) HL60, 8) rat brain and 9) mouse brain tissue lysate with VAMP4 antibody. Predicted molecular weight ~16 kDa.
5 / 5
Flow cytometry testing of human PC-3 cells with VAMP4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VAMP4 antibody.
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IHC staining of FFPE human breast cancer with VAMP4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with VAMP4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with VAMP4 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) U-87 MG, 2) T-47D, 3) K562, 4) PC-3, 5) ThP-1, 6) HEK293, 7) HL60, 8) rat brain and 9) mouse brain tissue lysate with VAMP4 antibody. Predicted molecular weight ~16 kDa.
Flow cytometry testing of human PC-3 cells with VAMP4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VAMP4 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the VAMP4 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Vesicle-associated membrane protein 4 is a protein that in humans is encoded by the VAMP4 gene. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-A118) was used as the immunogen for the VAMP4 antibody.
Limitation:
This VAMP4 antibody is available for research use only.
Localization:
Cytoplasmic (Glogi)
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O75379