CDC42 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5236
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5236-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the CDC42 antibody at -20oC.

Images

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Western blot testing of human 1) placenta, 2) U937, 3) HL60, 4) U-2 OS, 5) U-87 MG, 6) K562, 7) HeLa, 8) rat thymus, 9) rat lung, 10) mouse lung, 11) mouse intestine and 12) mouse SP2/0 lysate with CDC42 antibody. Predicted molecular weight ~21 kDa.
2 / 11
Western blot testing of human 1) U-87 MG, 2) K562 and 3) HeLa cell lysate with CDC42 antibody. Predicted molecular weight ~21 kDa.
3 / 11
Western blot testing of 1) rat thymus, 2) rat lung and 3) mouse SP20 lysate with CDC42 antibody. Predicted molecular weight ~21 kDa.
4 / 11
IHC staining of FFPE human breast cancer with CDC42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human breast cancer with CDC42 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human colon cancer with CDC42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human colon cancer with CDC42 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE mouse intestine with CDC42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
IHC staining of FFPE rat intestine with CDC42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 11
Flow cytometry testing of human SiHa cells with CDC42 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDC42 antibody.
11 / 11
Flow cytometry testing of human U-2 OS cells with CDC42 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDC42 antibody.
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Western blot testing of human 1) placenta, 2) U937, 3) HL60, 4) U-2 OS, 5) U-87 MG, 6) K562, 7) HeLa, 8) rat thymus, 9) rat lung, 10) mouse lung, 11) mouse intestine and 12) mouse SP2/0 lysate with CDC42 antibody. Predicted molecular weight ~21 kDa.
Western blot testing of human 1) U-87 MG, 2) K562 and 3) HeLa cell lysate with CDC42 antibody. Predicted molecular weight ~21 kDa.
Western blot testing of 1) rat thymus, 2) rat lung and 3) mouse SP20 lysate with CDC42 antibody. Predicted molecular weight ~21 kDa.
IHC staining of FFPE human breast cancer with CDC42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with CDC42 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with CDC42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon cancer with CDC42 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse intestine with CDC42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat intestine with CDC42 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human SiHa cells with CDC42 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDC42 antibody.
Flow cytometry testing of human U-2 OS cells with CDC42 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDC42 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CDC42 antibody should be determined by the researcher.
Description:
Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene. CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids K16-C188 from the human protein were used as the immunogen for the CDC42 antibody.
Limitation:
This CDC42 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P60953