Angiopoietin 2 Antibody / ANG2

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6037
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6037-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ANGPT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 7
Immunofluorescent staining of FFPE human A549 cells with ANGPT2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
Western blot testing of human 1) SW620, 2) A549, 3) K562, 4) rat brain and 5) mouse spleen lysate with ANGPT2 antibody. Expected molecular weight: 57-70 kDa depending on glycosylation level.
3 / 7
Flow cytometry testing of human U937 cells with ANGPT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ANGPT2 antibody.
4 / 7
Flow cytometry testing of human HeLa cells with ANGPT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ANGPT2 antibody.
5 / 7
IHC staining of FFPE human placenta with ANGPT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
IHC staining of FFPE human breast cancer with ANGPT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 7
IHC staining of FFPE human rectal cancer with ANGPT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
❮❯

Immunofluorescent staining of FFPE human A549 cells with ANGPT2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) SW620, 2) A549, 3) K562, 4) rat brain and 5) mouse spleen lysate with ANGPT2 antibody. Expected molecular weight: 57-70 kDa depending on glycosylation level.
Flow cytometry testing of human U937 cells with ANGPT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ANGPT2 antibody.
Flow cytometry testing of human HeLa cells with ANGPT2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ANGPT2 antibody.
IHC staining of FFPE human placenta with ANGPT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with ANGPT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with ANGPT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ANGPT2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
ANGPT2, also known as ANG2 or Angiopoietin 2, is a protein that in humans is encoded by the ANGPT2 gene. It is mapped to 8p23.1. ANGPT2 is a naturally occurring antagonist of ANG1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. ANGPT2 was significantly increased in plasma and alveolar edema fluid in adults with acute lung injury compared to controls or patients with hydrostatic pulmonary edema, tracheal. ANGPT2 was also significantly increased in neonates with respiratory distress syndrome who developed bronchopulmonary edema. It is also a mediator of epithelial necrosis with an important role in hyperoxic acute lung injury and pulmonary edema.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids N357-K404) was used as the immunogen for the ANGPT2 antibody.
Limitation:
This ANGPT2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O15123