CBX8 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5872
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5872-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CBX8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A549 cells with CBX8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE mouse small intestine with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
IHC staining of FFPE rat small intestine with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 8
Western blot testing of human 1) HeLa, 2) A549, 3) U-2 OS, 4) COLO-320, 5) Raji, 6) SW620, 7) rat PC-3 and 8) mouse SP2/0 lysate with CBX8 antibody. Predicted molecular weight ~43 kDa.
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Flow cytometry testing of human A549 cells with CBX8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CBX8 antibody.
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Immunofluorescent staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with CBX8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse small intestine with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat small intestine with CBX8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) A549, 3) U-2 OS, 4) COLO-320, 5) Raji, 6) SW620, 7) rat PC-3 and 8) mouse SP2/0 lysate with CBX8 antibody. Predicted molecular weight ~43 kDa.
Flow cytometry testing of human A549 cells with CBX8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CBX8 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Immunocytochemistry: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CBX8 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CBX8 functions as a transcriptional repressor and has a role in DNA damage response. This gene is mapped to chromosome 17q25.3 based on an alignment of the CBX8 sequence with the genomic sequence (GRCh38).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-R389) was used as the immunogen for the CBX8 antibody.
Limitation:
This CBX8 antibody is available for research use only.
Localization:
Nuclear
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9HC52