Glutamic-oxaloacetic transaminase 1 Antibody / GOT1

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NSJ Bioreagents
Product Code: NSJ-RQ6423
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6423-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Glutamic-oxaloacetic transaminase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human T-47D cells with Glutamic-oxaloacetic transaminase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE mouse brain tissue with Glutamic-oxaloacetic transaminase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat brain tissue with Glutamic-oxaloacetic transaminase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) placenta, 2) T-47D, 3) HepG2, 4) Caco-2, 5) HL60, 6) K562, 7) HeLa, 8) rat brain, 9) mouse brain and 10) mouse liver tissue lysate with Glutamic-oxaloacetic transaminase 1 antibody. Predicted molecular weight ~41 kDa.
5 / 5
Flow cytometry testing of human HepG2 cells with Glutamic-oxaloacetic transaminase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Aspartate Aminotransferase antibody.
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Immunofluorescent staining of FFPE human T-47D cells with Glutamic-oxaloacetic transaminase 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse brain tissue with Glutamic-oxaloacetic transaminase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Glutamic-oxaloacetic transaminase 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) placenta, 2) T-47D, 3) HepG2, 4) Caco-2, 5) HL60, 6) K562, 7) HeLa, 8) rat brain, 9) mouse brain and 10) mouse liver tissue lysate with Glutamic-oxaloacetic transaminase 1 antibody. Predicted molecular weight ~41 kDa.
Flow cytometry testing of human HepG2 cells with Glutamic-oxaloacetic transaminase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Aspartate Aminotransferase antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Glutamic-oxaloacetic transaminase 1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Aspartate aminotransferase, cytoplasmic is an enzyme that in humans is encoded by the GOT1 gene. Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids S5-Q413) was used as the immunogen for the Glutamic-oxaloacetic transaminase 1 antibody.
Limitation:
This Aspartate Aminotransferase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P17174