LSM8 Antibody

Product Code: NSJ-RQ6143

Code	Size	Price

NSJ-RQ6143-100ug

100 ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Fluorescence-activated cell sorting (FACS)
Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the LSM8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE rat testis with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

2 / 13

IHC staining of FFPE mouse testis with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

3 / 13

IHC staining of FFPE human tonsil with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

4 / 13

IHC staining of FFPE human ovarian cancer with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

5 / 13

IHC staining of FFPE human skin cancer with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

6 / 13

IHC staining of FFPE human appendicitis tissue with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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IHC staining of FFPE human gastric cancer with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

8 / 13

IHC staining of FFPE human melanoma tissue with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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IHC staining of FFPE human renal carcinoma with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

10 / 13

IHC staining of FFPE human bladder cancer with LSM8 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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Immunofluorescent staining of FFPE human A431 cells with LSM8 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

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Western blot testing of human 1) HeLa, 2) HL60, 3) HepG2, 4) U937, 5) Jurkat, 6) Raji, 7) ThP-1, 8) K562, 9) rat testis, 10) rat lung, 11) rat liver, 12) rat pancreas, 13) mouse testis, 14) mouse lung, 15) mouse liver and 16) mouse HEPA1-6 lysate with LSM8 antibody. Predicted molecular weight ~11 kDa.

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Flow cytometry testing of human A431 cells with LSM8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LSM8 antibody.

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Documents

Further Information

Application Details :

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

Application Note:

Optimal dilution of the LSM8 antibody should be determined by the researcher.

Description:

U6 snRNA-associated Sm-like protein LSm8 is a protein that in humans is encoded by the LSM8 gene. This gene encodes a member of the like-Sm family of proteins. The encoded protein consists of a closed barrel shape, made up of five anti-parallel beta strands and an alpha helix. This protein partners with six paralogs to form a heteroheptameric ring which transiently binds U6 small nuclear RNAs and is involved in the general maturation of RNA in the nucleus.

Format :

Antigen affinity purified

Formulation :

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Immunogen:

A human recombinant partial protein (amino acids M1-H96) was used as the immunogen for the LSM8 antibody.

Limitation:

This LSM8 antibody is available for research use only.

Localization:

Nuclear

Purity:

Affinity purified

Species Reactivity :

Human, Mouse, Rat

Uniprot #:

O95777