MIEF1 Antibody / Mitochondrial dynamics protein MID51

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6428
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6428-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the MIEF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human renal clear cell carcinoma tissue with MIEF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human rectal cancer tissue with MIEF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with MIEF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 5
Western blot testing of human 1) HT1080, 2) Jurkat, 3) K562, 4) HL60, 5) HEK293, 6) U937, 7) HepG2, 8) rat heart, 9) rat kidney and 10) mouse kidney tissue lysate with MIEF1 antibody. Predicted molecular weight ~51 kDa.
5 / 5
Flow cytometry testing of human SiHa cells with MIEF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MIEF1 antibody.
❮❯

IHC staining of FFPE human renal clear cell carcinoma tissue with MIEF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with MIEF1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with MIEF1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HT1080, 2) Jurkat, 3) K562, 4) HL60, 5) HEK293, 6) U937, 7) HepG2, 8) rat heart, 9) rat kidney and 10) mouse kidney tissue lysate with MIEF1 antibody. Predicted molecular weight ~51 kDa.
Flow cytometry testing of human SiHa cells with MIEF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MIEF1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the MIEF1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Mitochondrial dynamic protein MID51 (MID51) also known as mitochondrial elongation factor 1 (MIEF1) or Smith-Magenis syndrome chromosome region candidate gene 7 protein-like (SMCR7L) is a protein that in humans is encoded by the SMCR7L gene. The SMCR7L gene codes for a protein that has been called MiD51/MIEF1 and shown to regulate mitochondrial fission by interacting with the proteins Drp1 and FIS1.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids S189-T463) was used as the immunogen for the MIEF1 antibody.
Limitation:
This MIEF1 antibody is available for research use only.
Localization:
Cytoplasmic (mitochondria)
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9NQG6