SEC14L3 Antibody / TAP2

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NSJ Bioreagents
Product Code: NSJ-RQ6436
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6436-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SEC14L3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human rectal cancer tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human gallbladder adenocarcinoma tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human breast cancer tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human gastric cancer tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat testis tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
Immunofluorescent staining of FFPE human HeLa cells with SEC14L3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of 1) human HEK293, 2) human U-87 MG, 3) human PC-3, 4) rat lung, 5) rat liver, 6) mouse lung and 7) mouse liver tissue lysate with SEC14L3 antibody. Predicted molecular weight: 37-46 kDa (multiple isoforms).
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Flow cytometry testing of human U937 cells with SEC14L3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC14L3 antibody.
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IHC staining of FFPE human rectal cancer tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gallbladder adenocarcinoma tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis tissue with SEC14L3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human HeLa cells with SEC14L3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HEK293, 2) human U-87 MG, 3) human PC-3, 4) rat lung, 5) rat liver, 6) mouse lung and 7) mouse liver tissue lysate with SEC14L3 antibody. Predicted molecular weight: 37-46 kDa (multiple isoforms).
Flow cytometry testing of human U937 cells with SEC14L3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SEC14L3 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SEC14L3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The protein encoded by this gene is highly similar to the protein encoded by the Saccharomyces cerevisiae SEC14 gene. The SEC14 protein is a phophatidylinositol transfer protein that is essential for biogenesis of Golgi-derived transport vesicles, and thus is required for the export of yeast secretory proteins from the Golgi complex. The specific function of this protein has not yet been determined. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids R4-V400) was used as the immunogen for the SEC14L3 antibody.
Limitation:
This SEC14L3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UDX4