LBR Antibody / Lamin B Receptor

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NSJ Bioreagents
Product Code: NSJ-RQ5307
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5307-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store the LBR antibody at -20oC.

Images

1 / 5
Immunofluorescent staining of human U-2 OS cells with LBR antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human lung cancer with LBR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 5
Western blot testing of human 1) placenta, 2) HL-60, 3) K562, 4) ThP-1, 5) HEK293, 6) U-2 OS, 7) U937 and 8) Caco-2 lysate with LBR antibody. Predicted molecular weight ~71 kDa.
4 / 5
Western blot testing of rat 1) thymus, 2) spleen, 3) testis and mouse 4) thymus, 5) stomach, 6) testis and 7) SP2/0 lysate with LBR antibody. Predicted molecular weight ~71 kDa.
5 / 5
Flow cytometry testing of human U937 cells with LBR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LBR antibody.
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Immunofluorescent staining of human U-2 OS cells with LBR antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lung cancer with LBR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of human 1) placenta, 2) HL-60, 3) K562, 4) ThP-1, 5) HEK293, 6) U-2 OS, 7) U937 and 8) Caco-2 lysate with LBR antibody. Predicted molecular weight ~71 kDa.
Western blot testing of rat 1) thymus, 2) spleen, 3) testis and mouse 4) thymus, 5) stomach, 6) testis and 7) SP2/0 lysate with LBR antibody. Predicted molecular weight ~71 kDa.
Flow cytometry testing of human U937 cells with LBR antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LBR antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the LBR antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Lamin-B receptor is a protein, and in humans, it is encoded by the LBR gene. It is mapped to 1q42.12. The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids H102-F209 from the human protein were used as the immunogen for the LBR antibody.
Limitation:
This LBR antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q14739