ADAMTS5 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6310
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6310-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ADAMTS5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
IHC staining of FFPE human placental tissue with ADAMTS5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 2
Western blot testing of human 1) HeLa, 2) HepG2, 3) U-2 OS, 4) SW620, 5) ThP-1, 6) Raji, 7) SGC-7901, 8) rat liver, 9) rat testis, 10) mouse liver and 11) mouse testis lysate with ADAMTS5 antibody. Predicted molecular weight ~102 kDa, but the processed forms can be observed at approx. 50-102 kDa.
❮❯

IHC staining of FFPE human placental tissue with ADAMTS5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) HepG2, 3) U-2 OS, 4) SW620, 5) ThP-1, 6) Raji, 7) SGC-7901, 8) rat liver, 9) rat testis, 10) mouse liver and 11) mouse testis lysate with ADAMTS5 antibody. Predicted molecular weight ~102 kDa, but the processed forms can be observed at approx. 50-102 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ADAMTS5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
ADAMTS5 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 5), is an enzyme that in humans is encoded by the ADAMTS5 gene. ADAMTS5 is a member of the large ADAMTS family of zinc-dependent proteases. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. By somatic cell hybrid analysis, the human ADAMTS5 gene is mapped to chromosome 21. Used mouse models, it is showed that Sdc4 controls a pathway that activates Adamts5 at the chondrocyte cell surface through Erk1/Erk2 activation of Mmp3.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids D747-K780) was used as the immunogen for the ADAMTS5 antibody.
Limitation:
This ADAMTS5 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UNA0