CMBL Antibody / LOC134147

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NSJ Bioreagents
Product Code: NSJ-RQ6089
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6089-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CMBL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human lung cancer with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human colorectal cancer with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human adnexal serous adenocarcinoma tissue with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) K562, 2) PC-3, 3) HepG2, 4) HEK293, 5) A549, 6) U-87 MG, 7) rat liver, 8) rat kidney and 9) mouse kidney lysate with CMBL antibody. Predicted molecular weight ~28 kDa.
5 / 5
Flow cytometry testing of human U-2 OS cells with CMBL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CMBL antibody.
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IHC staining of FFPE human lung cancer with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal cancer with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human adnexal serous adenocarcinoma tissue with CMBL antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562, 2) PC-3, 3) HepG2, 4) HEK293, 5) A549, 6) U-87 MG, 7) rat liver, 8) rat kidney and 9) mouse kidney lysate with CMBL antibody. Predicted molecular weight ~28 kDa.
Flow cytometry testing of human U-2 OS cells with CMBL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CMBL antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CMBL antibody should be determined by the researcher.
Description:
CMBL is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL preferentially cleaves cyclic esters, and it activates medoxomil-ester prodrugs in which the medoxomil moiety is linked to an oxygen atom.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids M1-M246) was used as the immunogen for the CMBL antibody.
Limitation:
This CMBL antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q96DG6