GRK2 Antibody / Beta-adrenergic receptor kinase 1

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NSJ Bioreagents
Product Code: NSJ-RQ5815
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5815-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the B-ARK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
Flow cytometry testing of human U937 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.
2 / 5
Flow cytometry testing of mouse HEPA1-6 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.
3 / 5
Flow cytometry testing of rat RH35 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.
4 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with B-ARK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 5
Western blot testing of 1) human ThP-1, 2) monkey COS-7, 3) human Raji, 4) rat lung, 5) mouse NIH 3T3 and 6) mouse RAW264.7 lysate with B-ARK1 antibody. Predicted molecular weight ~79 kDa.
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Flow cytometry testing of human U937 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.
Flow cytometry testing of mouse HEPA1-6 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.
Flow cytometry testing of rat RH35 cells with B-ARK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-ARK1 antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with B-ARK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human ThP-1, 2) monkey COS-7, 3) human Raji, 4) rat lung, 5) mouse NIH 3T3 and 6) mouse RAW264.7 lysate with B-ARK1 antibody. Predicted molecular weight ~79 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the B-ARK1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Beta adrenergic receptor kinase (beta-ARK or BARK) is a serine/threonine intracellular kinase. The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids KKELRDAYREAQQLVQRV from the human protein were used as the immunogen for the B-ARK1 antibody.
Limitation:
This B-ARK1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
P25098