ACADSB Antibody / SBCAD

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NSJ Bioreagents
Product Code: NSJ-RQ5791
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5791-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the ACADSB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE human MCF7 cells with ACADSB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
Flow cytometry testing of human ThP-1 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.
3 / 5
Flow cytometry testing of mouse RAW264.7 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.
4 / 5
Flow cytometry testing of rat RH-35 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.
5 / 5
Western blot testing of 1) human A431, 2) human T-47 D, 3) human A549, 4) monkey COS-7, 5) rat liver and 6) mouse liver lysate with ACADSB antibody. Expected molecular weight: ~47 kDa (unprocessed), ~43 kDa (processed).
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Immunofluorescent staining of FFPE human MCF7 cells with ACADSB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human ThP-1 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.
Flow cytometry testing of mouse RAW264.7 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.
Flow cytometry testing of rat RH-35 cells with ACADSB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACADSB antibody.
Western blot testing of 1) human A431, 2) human T-47 D, 3) human A549, 4) monkey COS-7, 5) rat liver and 6) mouse liver lysate with ACADSB antibody. Expected molecular weight: ~47 kDa (unprocessed), ~43 kDa (processed).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the ACADSB antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
ACADSB is a human gene that encodes short/branched chain specific acyl-CoA dehydrogenase (SBCAD), an enzyme in the acyl CoA dehydrogenase family. Short/branched chain acyl-CoA dehydrogenase (ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids ASNIQLNTIAKHIDAEY from the human protein were used as the immunogen for the ACADSB antibody.
Limitation:
This ACADSB antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
P45954