RAB1B Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6207
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6207-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the RAB1B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 9
IHC staining of FFPE human breast cancer with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate cancer with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 9
IHC staining of FFPE human ovarian cancer with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 9
IHC staining of FFPE human tonsil with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 9
IHC staining of FFPE human placenta with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 9
Immunofluorescent staining of FFPE human T-47D cells with RAB1B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 9
Western blot testing of human 1) HeLa, 2) HEK293, 3) SK-OV-3, 4) MCF7, 5) A375, 6) U-87 MG, 7) A549, 8) rat kidney, 9) rat brain, 10) rat heart, 11) rat NRK, 12) mouse brain and 13) mouse heart lysate with RAB1B antibody. Predicted molecular weight ~24 kDa.
8 / 9
Flow cytometry testing of human U-251 cells with RAB1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB1B antibody.
9 / 9
Flow cytometry testing of mouse HEPA1-6 cells with RAB1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB1B antibody.
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IHC staining of FFPE human breast cancer with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate cancer with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian cancer with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with RAB1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human T-47D cells with RAB1B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) HEK293, 3) SK-OV-3, 4) MCF7, 5) A375, 6) U-87 MG, 7) A549, 8) rat kidney, 9) rat brain, 10) rat heart, 11) rat NRK, 12) mouse brain and 13) mouse heart lysate with RAB1B antibody. Predicted molecular weight ~24 kDa.
Flow cytometry testing of human U-251 cells with RAB1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB1B antibody.
Flow cytometry testing of mouse HEPA1-6 cells with RAB1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB1B antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the RAB1B antibody should be determined by the researcher.
Description:
Ras-related protein Rab-1B is a protein that in humans is encoded by the RAB1B gene. Members of the RAB protein family, such as RAB1B, are low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB1B functions in the early secretory pathway and is essential for vesicle transport between the endoplasmic reticulum (ER) and Golgi.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids AAEIKKRMGPGAASGGERPNLKIDSTPVKPA from the human protein were used as the immunogen for the RAB1B antibody.
Limitation:
This RAB1B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
Q9H0U4