RAPGEF3 Antibody / Epac1

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NSJ Bioreagents
Product Code: NSJ-RQ6153
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6153-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the RAPGEF3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human U-87 MG cells with RAPGEF3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAPGEF3 antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human placenta, 3) monkey kidney, 4) rat kidney, 5) rat lung and 6) mouse kidney lysate with RAPGEF3 antibody. Predicted molecular weight ~104 kDa.
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Flow cytometry testing of human U-87 MG cells with RAPGEF3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAPGEF3 antibody.
Western blot testing of 1) human HeLa, 2) human placenta, 3) monkey kidney, 4) rat kidney, 5) rat lung and 6) mouse kidney lysate with RAPGEF3 antibody. Predicted molecular weight ~104 kDa.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RAPGEF3 antibody should be determined by the researcher.
Description:
Rap guanine nucleotide exchange factor 3 also known as exchange factor directly activated by cAMP 1 (EPAC1) or cAMP-regulated guanine nucleotide exchange factor I (cAMP-GEFI) is a protein that in humans is encoded by the RAPGEF3 gene. As the name suggests, EPAC proteins (EPAC1 and EPAC2) are a family of intracellular sensors for cAMP, and function as nucleotide exchange factors for the Rap subfamily of RAS-like small GTPases.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids E451-D698) was used as the immunogen for the RAPGEF3 antibody.
Limitation:
This RAPGEF3 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
O95398