NNT Antibody / Nicotinamide Nucleotide Transhydrogenase

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NSJ Bioreagents
Product Code: NSJ-RQ5901
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5901-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the NNT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with NNT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) rat liver, 2) rat kidney, 3) rat heart, 4) mouse liver, 5) mouse kidney, 6) mouse heart, 7) human HeLa, 8) monkey liver and 9) monkey kidney lysate with NNT antibody. Predicted molecular weight ~114 kDa.
3 / 3
Flow cytometry testing of human ThP-1 cells with NNT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NNT antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with NNT antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat liver, 2) rat kidney, 3) rat heart, 4) mouse liver, 5) mouse kidney, 6) mouse heart, 7) human HeLa, 8) monkey liver and 9) monkey kidney lysate with NNT antibody. Predicted molecular weight ~114 kDa.
Flow cytometry testing of human ThP-1 cells with NNT antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NNT antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the NNT antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids C44-A350) was used as the immunogen for the NNT antibody.
Limitation:
This NNT antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
Q13423