USP25 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6017
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6017-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
After reconstitution, the USP25 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human A549 cells with USP25 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HEK293, 2) human placenta, 3) monkey liver, 4) monkey kidney, 5) human PC-3, 6) human HeLa, 7) human A549, 8) rat kidney, 9) rat liver, 10) rat lung, 11) rat PC-12, 12) mouse lung, 13) mouse RAW264.7 and 14) mouse NIH 3T3 lysate with USP25 antibody. Predicted molecular weight ~122 kDa.
3 / 3
Flow cytometry testing of human HL60 cells with USP25 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= USP25 antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with USP25 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HEK293, 2) human placenta, 3) monkey liver, 4) monkey kidney, 5) human PC-3, 6) human HeLa, 7) human A549, 8) rat kidney, 9) rat liver, 10) rat lung, 11) rat PC-12, 12) mouse lung, 13) mouse RAW264.7 and 14) mouse NIH 3T3 lysate with USP25 antibody. Predicted molecular weight ~122 kDa.
Flow cytometry testing of human HL60 cells with USP25 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= USP25 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the USP25 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Ubiquitin specific peptidase 25 is a protein that in humans is encoded by the USP25 gene. It is mapped to 21q21.1. Ubiquitin is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-D1053) was used as the immunogen for the USP25 antibody.
Limitation:
This USP25 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
Q9UHP3