CACNA1S Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6164
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6164-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CACNA1S antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human skeletal muscle with CACNA1S antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat skeletal muscle with CACNA1S antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) monkey skeletal muscle, 2) rat skeletal muscle and 3) mouse skeletal muscle lysate with CACNA1S antibody. Predicted molecular weight ~220 kDa.
4 / 4
Flow cytometry testing of human U937 cells with CACNA1S antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CACNA1S antibody.
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IHC staining of FFPE human skeletal muscle with CACNA1S antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat skeletal muscle with CACNA1S antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) monkey skeletal muscle, 2) rat skeletal muscle and 3) mouse skeletal muscle lysate with CACNA1S antibody. Predicted molecular weight ~220 kDa.
Flow cytometry testing of human U937 cells with CACNA1S antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CACNA1S antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CACNA1S antibody should be determined by the researcher.
Description:
Cav1.1 also known as the calcium channel, voltage-dependent, L type, alpha 1S subunit, (CACNA1S), is a protein which in humans is encoded by the CACNA1S gene. This gene encodes one of the five subunits of the slowly inactivating L-type voltage-dependent calcium channel in skeletal muscle cells. Mutations in this gene have been associated with hypokalemic periodic paralysis, thyrotoxic periodic paralysis and malignant hyperthermia susceptibility.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A human recombinant partial protein (amino acids E1583-C1779) was used as the immunogen for the CACNA1S antibody.
Limitation:
This CACNA1S antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
Q13698