Indoleamine 2,3-dioxygenase 2 Antibody / IDO2 / INDOL1

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NSJ Bioreagents
Product Code: NSJ-RQ6383
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6383-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Indoleamine 2,3-dioxygenase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE human liver cancer with Indoleamine 2,3-dioxygenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) rat liver, 2) mouse liver and 3) monkey kidney tissue lysate with Indoleamine 2,3-dioxygenase 2 antibody. Predicted molecular weight ~47 kDa.
3 / 3
Flow cytometry testing of human K562 cells with Indoleamine 2,3-dioxygenase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Indoleamine 2,3-dioxygenase 2 antibody.
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IHC staining of FFPE human liver cancer with Indoleamine 2,3-dioxygenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat liver, 2) mouse liver and 3) monkey kidney tissue lysate with Indoleamine 2,3-dioxygenase 2 antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human K562 cells with Indoleamine 2,3-dioxygenase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Indoleamine 2,3-dioxygenase 2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Indoleamine 2,3-dioxygenase 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
IDO2 (Indoleamine 2,3-dioxygenase 2), also called INDOLEAMINE 2,3-DIOXYGENASE-LIKE 1 or INDOL1, is an enzyme encoded by the INDOL1 gene which metabolizes tryptophan in the kynurenine pathway. By genomic sequence analysis, the INDOL1 gene is mapped on chromosome 8p12 just downstream of the INDO gene. And its exact cytogenetic location is 8p11.21. By database analysis using INDO as probe, followed by RT-PCR of total RNA from various tissues, IDO2 is cloned by human and mouse INDOL1. INDOL1 catabolizes tryptophan as determined by Kyn production, but unlike INDO, is inhibited by D-1-methyl-tryptophan(D-1MT) but not the L-1MT stereoisomer. The Gene Structure of the INDOL1 has 11 exons and spans 74 kb.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-R357) was used as the immunogen for the Indoleamine 2,3-dioxygenase 2 antibody.
Limitation:
This Indoleamine 2,3-dioxygenase 2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
Q6ZQW0