ACTR2 Antibody / Arp2

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NSJ Bioreagents
Product Code: NSJ-RQ5865
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5865-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the ACTR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human breast cancer with ACTR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
Immunofluorescent staining of FFPE human A549 cells with ACTR2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
Western blot testing of 1) rat kidney, 2) rat spleen, 3) mouse HEPA1-6, 4) mouse SP2/0, 5) monkey COS-7 and human 6) U-87 MG, 7) Jurkat, 8) PC-3 and 9) U-2 OS lysate with ACTR2 antibody. Predicted molecular weight ~45 kDa.
4 / 5
Flow cytometry testing of human A431 cells with ACTR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTR2 antibody.
5 / 5
Flow cytometry testing of mouse ANA-1 cells with ACTR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTR2 antibody.
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IHC staining of FFPE human breast cancer with ACTR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with ACTR2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat kidney, 2) rat spleen, 3) mouse HEPA1-6, 4) mouse SP2/0, 5) monkey COS-7 and human 6) U-87 MG, 7) Jurkat, 8) PC-3 and 9) U-2 OS lysate with ACTR2 antibody. Predicted molecular weight ~45 kDa.
Flow cytometry testing of human A431 cells with ACTR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTR2 antibody.
Flow cytometry testing of mouse ANA-1 cells with ACTR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTR2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the ACTR2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-R394) was used as the immunogen for the ACTR2 antibody.
Limitation:
This ACTR2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat, Monkey
Uniprot #:
P61160