LDLR Antibody / LDL Receptor

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NSJ Bioreagents
Product Code: NSJ-RQ5984
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5984-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the LDL Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human rectal cancer with LDL Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human liver cancer with LDL Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
Western blot testing of 1) human HeLa, 2) human Raji, 3) human U-87 MG, 4) rat lung, 5) rat kidney and 6) rat RH35 lysate with LDL Receptor antibody. Expected molecular weight: 95-160 kDa depending on glycosylation level.
4 / 5
Flow cytometry testing of human A431 cells with LDL Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDL Receptor antibody.
5 / 5
Flow cytometry testing of human NRK cells with LDL Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDL Receptor antibody.
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IHC staining of FFPE human rectal cancer with LDL Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with LDL Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human Raji, 3) human U-87 MG, 4) rat lung, 5) rat kidney and 6) rat RH35 lysate with LDL Receptor antibody. Expected molecular weight: 95-160 kDa depending on glycosylation level.
Flow cytometry testing of human A431 cells with LDL Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDL Receptor antibody.
Flow cytometry testing of human NRK cells with LDL Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDL Receptor antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the LDL Receptor antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
In humans, the LDL receptor protein is encoded by the LDLR gene on chromosome 19. It is mapped to 19p13.2. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids Q35-D843) was used as the immunogen for the LDL Receptor antibody.
Limitation:
This LDL Receptor antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P01130