CASP7 Antibody / Caspase-7

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NSJ Bioreagents
Product Code: NSJ-RQ6315
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6315-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the CASP7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
IHC staining of FFPE human rectal cancer with CASP7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human gastric cancer with CASP7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human breast cancer with CASP7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human lung cancer with CASP7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) Jurkat, 2) HEK293, 3) MCF7, 4) T47D, 5) A549 and 6) rat liver lysate with CASP7 antibody. Expected molecular weight ~34 kDa (full length).
6 / 6
Flow cytometry testing of human MCF7 cells with CASP7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CASP7 antibody.
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IHC staining of FFPE human rectal cancer with CASP7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human gastric cancer with CASP7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with CASP7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with CASP7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Jurkat, 2) HEK293, 3) MCF7, 4) T47D, 5) A549 and 6) rat liver lysate with CASP7 antibody. Expected molecular weight ~34 kDa (full length).
Flow cytometry testing of human MCF7 cells with CASP7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CASP7 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CASP7 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Caspase-7 is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A24-Q303) was used as the immunogen for the CASP7 antibody.
Limitation:
This CASP7 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P55210