Cd11c Antibody / Itgax

NSJ Bioreagents
Product Code: NSJ-RQ6137
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6137-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Itgax antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of mouse spleen cells with Itgax antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Itgax antibody.
2 / 2
Western blot testing of mouse 1) spleen and 2) lung tissue lysate with Itgax antibody. Expected molecular weight: 128-150 kDa.

Flow cytometry testing of mouse spleen cells with Itgax antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Itgax antibody.
Western blot testing of mouse 1) spleen and 2) lung tissue lysate with Itgax antibody. Expected molecular weight: 128-150 kDa.

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Itgax antibody should be determined by the researcher.
Description:
CD11c, also known as Integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX), is a genethat encodes for CD11c. This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A mouse recombinant partial protein (amino acids F20-E332) was used as the immunogen for the Itgax antibody.
Limitation:
This Itgax antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse
Uniprot #:
Q9QXH4