Mt-nd2 Antibody / NADH2

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NSJ Bioreagents
Product Code: NSJ-RQ5993
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5993-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Mt-nd2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of mouse ANA-1 cells with Mt-nd2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mt-nd2 antibody.
2 / 3
Flow cytometry testing of rat NRK cells with Mt-nd2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mt-nd2 antibody.
3 / 3
Western blot testing of 1) rat thymus, 2) rat spleen, 3) mouse thymus and 4) mouse HEPA1-6 lysate with Mt-nd2 antibody. Predicted molecular weight ~39 kDa.
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Flow cytometry testing of mouse ANA-1 cells with Mt-nd2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mt-nd2 antibody.
Flow cytometry testing of rat NRK cells with Mt-nd2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Mt-nd2 antibody.
Western blot testing of 1) rat thymus, 2) rat spleen, 3) mouse thymus and 4) mouse HEPA1-6 lysate with Mt-nd2 antibody. Predicted molecular weight ~39 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Mt-nd2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Mitochondrially encoded NADH dehydrogenase 2 is protein that in humans is encoded by the mitochondrial gene MT-ND2 gene. The ND2 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain. Variants of MT-ND2 are associated with mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS), Leigh's syndrome (LS), Leber's hereditary optic neuropathy (LHON) and increases in adult BMI.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids LEFSLLAIIPMLINKKNPRS from the mouse protein were used as the immunogen for the Mt-nd2 antibody.
Limitation:
This Mt-nd2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P03893