BCMA Antibody / Cd269 / Tnfrsf17

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NSJ Bioreagents
Product Code: NSJ-RQ5844
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5844-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Tnfrsf17 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 5
Flow cytometry testing of mouse HEPA1-6 cells with Tnfrsf17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tnfrsf17 antibody.
2 / 5
Flow cytometry testing of mouse RAW264.7 cells with Tnfrsf17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tnfrsf17 antibody.
3 / 5
Western blot testing of 1) mouse RAW264.7, 2) rat plasma and 3) mouse plasma lysate with Tnfrsf17 antibody. Expected molecular weight: 20-27 kDa depending on glycosylation level.
4 / 5
IHC staining of FFPE mouse spleen with Tnfrsf17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 5
IHC staining of FFPE rat spleen with Tnfrsf17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Flow cytometry testing of mouse HEPA1-6 cells with Tnfrsf17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tnfrsf17 antibody.
Flow cytometry testing of mouse RAW264.7 cells with Tnfrsf17 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tnfrsf17 antibody.
Western blot testing of 1) mouse RAW264.7, 2) rat plasma and 3) mouse plasma lysate with Tnfrsf17 antibody. Expected molecular weight: 20-27 kDa depending on glycosylation level.
IHC staining of FFPE mouse spleen with Tnfrsf17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat spleen with Tnfrsf17 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunohistochemistry (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the Tnfrsf17 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids FHSEYFDSLLHACKPCHLRC from the mouse protein were used as the immunogen for the Tnfrsf17 antibody.
Limitation:
This Tnfrsf17 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
O88472