Cfh Antibody / Complement factor H

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NSJ Bioreagents
Product Code: NSJ-RQ5805
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5805-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cfh antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE mouse liver with Cfh antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) mouse liver and 2) rat liver lysate with Cfh antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.
3 / 3
Flow cytometry testing of mouse HEPA1-6 cells with Cfh antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cfh antibody.
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IHC staining of FFPE mouse liver with Cfh antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) mouse liver and 2) rat liver lysate with Cfh antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.
Flow cytometry testing of mouse HEPA1-6 cells with Cfh antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cfh antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Cfh antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Factor H is a member of the regulators of complement activation family and is a complement control protein. This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids N136-T447) was used as the immunogen for the Cfh antibody.
Limitation:
This Cfh antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P06909