Cox8a Antibody

NSJ Bioreagents
Product Code: NSJ-RQ6090
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6090-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cox8a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE rat testis with Cox8a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) rat brain, 2) rat stomach, 3) rat kidney, 4) mouse brain and 5) mouse kidney lysate with Cox8a antibody. Predicted molecular weight ~8 kDa.
3 / 3
Flow cytometry testing of mouse spleen cells with Cox8a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cox8a antibody.

IHC staining of FFPE rat testis with Cox8a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain, 2) rat stomach, 3) rat kidney, 4) mouse brain and 5) mouse kidney lysate with Cox8a antibody. Predicted molecular weight ~8 kDa.
Flow cytometry testing of mouse spleen cells with Cox8a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cox8a antibody.

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Cox8a antibody should be determined by the researcher.
Description:
The protein encoded by this gene is the terminal enzyme of the respiratory chain, coupling the transfer of electrons from cytochrome c to molecular oxygen, with the concomitant production of a proton electrochemical gradient across the inner mitochondrial membrane. In addition to 3 mitochondrially encoded subunits, which perform the catalytic function, the eukaryotic enzyme contains nuclear-encoded smaller subunits, ranging in number from 4 in some organisms to 10 in mammals. It has been proposed that nuclear-encoded subunits may be involved in the modulation of the catalytic function. This gene encodes one of the nuclear-encoded subunits.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids AGWILSHLETYRRPE from the mouse protein were used as the immunogen for the Cox8a antibody.
Limitation:
This Cox8a antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q64445