Fadd Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6174
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6174-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Fadd antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of rat 1) brain, 2) stomach, 3) lung, 4) liver, 5) kidney, 6) spleen, 7) thymus and 8) PC-12 lysate with Fadd antibody. Predicted molecular weight ~23 kDa, commonly observed at 23-28 kDa.
2 / 4
Western blot testing of mouse 1) brain, 2) stomach, 3) lung, 4) liver, 5) kidney, 6) spleen, 7) thymus and 8) NIH 3T3 lysate with Fadd antibody. Predicted molecular weight ~23 kDa, commonly observed at 23-28 kDa.
3 / 4
IHC staining of FFPE mouse liver with Fadd antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
Flow cytometry testing of mouse HEPA1-6 cells with Fadd antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fadd antibody.
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Western blot testing of rat 1) brain, 2) stomach, 3) lung, 4) liver, 5) kidney, 6) spleen, 7) thymus and 8) PC-12 lysate with Fadd antibody. Predicted molecular weight ~23 kDa, commonly observed at 23-28 kDa.
Western blot testing of mouse 1) brain, 2) stomach, 3) lung, 4) liver, 5) kidney, 6) spleen, 7) thymus and 8) NIH 3T3 lysate with Fadd antibody. Predicted molecular weight ~23 kDa, commonly observed at 23-28 kDa.
IHC staining of FFPE mouse liver with Fadd antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of mouse HEPA1-6 cells with Fadd antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fadd antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Fadd antibody should be determined by the researcher.
Description:
FADD, Fas-Associated protein with Death Domain, is a universal adaptor protein in apoptosis that mediates signaling of all known death domain-containing members of the TNF receptor superfamily. The FADD gene contains 2 exons and spans approximately 3.6 kb. By analysis of somatic cell hybrid panels and by fluorescence in situ hybridization, the FADD gene is mapped to 11q13.3. The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, thus, it participates in the death signaling initiated by these receptors.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids AEKKNASVAGLVKALRTCRLNLVADLVEEAQES from the mouse protein were used as the immunogen for the Fadd antibody.
Limitation:
This Fadd antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q61160