Caspase-9 Antibody (p35)

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NSJ Bioreagents
Product Code: NSJ-RQ5798
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5798-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Caspase 9 p35 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) mouse heart, 2) rat heart and 3) rat skeletal muscle with Caspase 9 p35 antibody.
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Flow cytometry testing of mouse spleen cells with Caspase 9 p35 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase 9 p35 antibody.
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Western blot testing of 1) mouse heart, 2) rat heart and 3) rat skeletal muscle with Caspase 9 p35 antibody.
Flow cytometry testing of mouse spleen cells with Caspase 9 p35 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Caspase 9 p35 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Caspase 9 p35 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Caspase 9 is an enzyme that in humans is encoded by the CASP9 gene. This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids E3-D266) was used as the immunogen for the Caspase 9 p35 antibody.
Limitation:
This Caspase 9 p35 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q8C3Q9