Fas Antibody / CD95

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NSJ Bioreagents
Product Code: NSJ-RQ6052
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6052-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Fas antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of mouse spleen cells with Fas antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fas antibody.
2 / 3
Flow cytometry testing of mouse RAW264.7 cells with Fas antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fas antibody.
3 / 3
Western blot testing of 1) rat thymus and 2) mouse RAW264.7 cell lysate with Fas antibody. Predicted molecular weight: 35-38 kDa (unmodified), 40-55 kDa (glycosylated).
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Flow cytometry testing of mouse spleen cells with Fas antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fas antibody.
Flow cytometry testing of mouse RAW264.7 cells with Fas antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Fas antibody.
Western blot testing of 1) rat thymus and 2) mouse RAW264.7 cell lysate with Fas antibody. Predicted molecular weight: 35-38 kDa (unmodified), 40-55 kDa (glycosylated).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Fas antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
FAS (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. FAS antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and it is mapped to 10q24.1. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signaling pathway has been shown to participate in immune escape and tumorigenesis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids E46-K279) was used as the immunogen for the Fas antibody.
Limitation:
This Fas antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P25446