RNase L Antibody / Rnasel

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NSJ Bioreagents
Product Code: NSJ-RQ6435
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6435-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the RNase L antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Flow cytometry testing of mouse HEPA1-6 cells with RNase L antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNase L antibody.
2 / 3
Flow cytometry testing of rat NRK cells with RNase L antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNase L antibody.
3 / 3
Western blot testing of mouse 1) lung and 2) RAW264.7 cell lysate with RNase L antibody. Predicted molecular weight ~83 kDa.
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Flow cytometry testing of mouse HEPA1-6 cells with RNase L antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNase L antibody.
Flow cytometry testing of rat NRK cells with RNase L antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNase L antibody.
Western blot testing of mouse 1) lung and 2) RAW264.7 cell lysate with RNase L antibody. Predicted molecular weight ~83 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RNase L antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
RNase L is an enzyme that in humans is encoded by the RNASEL gene. This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids E24-S735) was used as the immunogen for the RNase L antibody.
Limitation:
This RNase L antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q05921