RNF8 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5760
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5760-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the RNF8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of mouse RAW264.7 cells with RNF8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNF8 antibody.
2 / 2
Western blot testing of 1) rat brain, 2) mouse brain and 3) NIH 3T3 lysate with RNF8 antibody. Predicted molecular weight ~55 kDa.
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Flow cytometry testing of mouse RAW264.7 cells with RNF8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RNF8 antibody.
Western blot testing of 1) rat brain, 2) mouse brain and 3) NIH 3T3 lysate with RNF8 antibody. Predicted molecular weight ~55 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RNF8 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
E3 ubiquitin-protein ligase RNF8 is an enzyme that in humans is encoded by the RNF8 gene. The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids E3-S488) was used as the immunogen for the RNF8 antibody.
Limitation:
This RNF8 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q8VC56