Afm Antibody / Afamin

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NSJ Bioreagents
Product Code: NSJ-RQ5793
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5793-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Afm antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE rat liver with Afm antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
Flow cytometry testing of mouse spleen cells with Afm antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Afm antibody.
3 / 4
Flow cytometry testing of mouse HEPA1-6 cells with Afm antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Afm antibody.
4 / 4
Western blot testing of rat 1) heart, 2) liver, 3) kidney, 4) spleen and mouse 5) spleen, 6) thymus and 7) ANA-1 lysate with Afm antibody. Predicted molecular weight: ~69/87 kDa (unmodified/glycosylated).
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IHC staining of FFPE rat liver with Afm antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of mouse spleen cells with Afm antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Afm antibody.
Flow cytometry testing of mouse HEPA1-6 cells with Afm antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Afm antibody.
Western blot testing of rat 1) heart, 2) liver, 3) kidney, 4) spleen and mouse 5) spleen, 6) thymus and 7) ANA-1 lysate with Afm antibody. Predicted molecular weight: ~69/87 kDa (unmodified/glycosylated).

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Afm antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally-related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids D40-K596) was used as the immunogen for the Afm antibody.
Limitation:
This Afm antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
O89020