Cd72 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6087
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6087-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cd72 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE mouse HEPA1-6 cells with Cd72 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
IHC staining of FFPE mouse spleen with Cd72 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE rat spleen with Cd72 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Flow cytometry testing of mouse spleen cells with Cd72 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd72 antibody.
5 / 6
Flow cytometry testing of mouse ANA-1 cells with Cd72 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd72 antibody.
6 / 6
Western blot testing of 1) rat liver and 2) mouse liver lysate with Cd72 antibody. Expected molecular weight: 39-45 kDa (monomer), ~86 kDa (dimer).
❮❯

Immunofluorescent staining of FFPE mouse HEPA1-6 cells with Cd72 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE mouse spleen with Cd72 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat spleen with Cd72 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of mouse spleen cells with Cd72 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd72 antibody.
Flow cytometry testing of mouse ANA-1 cells with Cd72 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd72 antibody.
Western blot testing of 1) rat liver and 2) mouse liver lysate with Cd72 antibody. Expected molecular weight: 39-45 kDa (monomer), ~86 kDa (dimer).

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Cd72 antibody should be determined by the researcher.
Description:
CD72 (Cluster of Differentiation 72), also known in murine biology as Lyb-2, is a protein active in the immune systemof animals. This gene is mapped to 9p13.3. It consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells, where it appears to mediate aspects of B-cell - T-cell interaction. It is a ligand for CD5.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A mouse recombinant partial protein (amino acids A8-R346) was used as the immunogen for the Cd72 antibody.
Limitation:
This Cd72 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P21855