Il1ra Antibody / Interleukin-1 receptor antagonist / Il1rn

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NSJ Bioreagents
Product Code: NSJ-RQ6425
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6425-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Il1ra antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of rat 1) spleen, 2) brain, 3) thymus, 4) kidney and mouse 5) spleen, 6) kidney, 7) RAW264.7 and 8) NIH 3T3 cell lysate with Il1ra antibody. Predicted molecular weight ~20 kDa.
2 / 3
Flow cytometry testing of mouse HEPA1-6 cells with Il1ra antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il1ra antibody.
3 / 3
Flow cytometry testing of rat NRK cells with Il1ra antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il1ra antibody.
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Western blot testing of rat 1) spleen, 2) brain, 3) thymus, 4) kidney and mouse 5) spleen, 6) kidney, 7) RAW264.7 and 8) NIH 3T3 cell lysate with Il1ra antibody. Predicted molecular weight ~20 kDa.
Flow cytometry testing of mouse HEPA1-6 cells with Il1ra antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il1ra antibody.
Flow cytometry testing of rat NRK cells with Il1ra antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il1ra antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Il1ra antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids K35-Q178) was used as the immunogen for the Il1ra antibody.
Limitation:
This Il1ra antibody is available for research use only.
Localization:
Cytoplasmic, secreted
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P25085