Il-3rb Antibody / Csf2rb / Cd131

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NSJ Bioreagents
Product Code: NSJ-RQ5968
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5968-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Il-3rb antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE rat intestine with Il-3rb antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
Western blot testing of mouse ANA-1 cells with Il-3rb antibody. Expected molecular weight: 99-130 kDa depending on glycosylation level.
3 / 4
Flow cytometry testing of mouse ANA-1 cells with Il-3rb antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il-3rb antibody.
4 / 4
Flow cytometry testing of rat NRK cells with Il-3rb antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il-3rb antibody.
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IHC staining of FFPE rat intestine with Il-3rb antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse ANA-1 cells with Il-3rb antibody. Expected molecular weight: 99-130 kDa depending on glycosylation level.
Flow cytometry testing of mouse ANA-1 cells with Il-3rb antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il-3rb antibody.
Flow cytometry testing of rat NRK cells with Il-3rb antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il-3rb antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Il-3rb antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Cytokine receptor common subunit beta is a protein that in humans is encoded by the CSF2RB gene. It is mapped to 15 E1; 15 37.36 cM. The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP).
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids A28-Q891) was used as the immunogen for the Il-3rb antibody.
Limitation:
This Il-3rb antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P26955