Ly6c Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ5893
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5893-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Ly6c antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE rat spleen with Ly6c antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of mouse 1) RAW264.7 and 2) ANA-1 cell lysate with Ly6c antibody. Predicted molecular weight ~14 kDa.
3 / 3
Flow cytometry testing of mouse spleen cells with Ly6c antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ly6c antibody.
❮❯

IHC staining of FFPE rat spleen with Ly6c antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse 1) RAW264.7 and 2) ANA-1 cell lysate with Ly6c antibody. Predicted molecular weight ~14 kDa.
Flow cytometry testing of mouse spleen cells with Ly6c antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ly6c antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Ly6c antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Ly6C is a murine cell-surface antigen expressed by plasma cells, subsets of myeloid cells and many T cells, including memory T cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids L27-G109) was used as the immunogen for the Ly6c antibody.
Limitation:
This Ly6c antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P0CW02