Pycard Antibody / Tms1 / mAsc

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NSJ Bioreagents
Product Code: NSJ-RQ6434
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6434-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Pycard antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE mouse intestine tissue with Pycard antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) rat lung, 2) rat spleen, 3) rat thymus, 4) mouse lung, 5) mouse spleen and 6) mouse thymus tissue lysate with Pycard antibody. Predicted molecular weight ~22 kDa.
3 / 3
Flow cytometry testing of mouse RAW264.7 cells with Pycard antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pycard antibody.
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IHC staining of FFPE mouse intestine tissue with Pycard antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat lung, 2) rat spleen, 3) rat thymus, 4) mouse lung, 5) mouse spleen and 6) mouse thymus tissue lysate with Pycard antibody. Predicted molecular weight ~22 kDa.
Flow cytometry testing of mouse RAW264.7 cells with Pycard antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Pycard antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Pycard antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids R3-S193) was used as the immunogen for the Pycard antibody.
Limitation:
This Pycard antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q9EPB4