Rad9b Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5916
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5916-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Rad9b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE rat brain with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 7
IHC staining of FFPE rat brain with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 7
IHC staining of FFPE rat heart with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 7
IHC staining of FFPE mouse heart with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 7
Western blot testing of 1) rat RH35, 2) mouse NIH 3T3 and 3) mouse SP2/0 lysate with Rad9b antibody. Expected molecular weight: 46-60 kDa.
7 / 7
Flow cytometry testing of mouse HEPA1-6 cells with Rad9b antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Rad9b antibody.
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IHC staining of FFPE rat brain with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat heart with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse heart with Rad9b antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat RH35, 2) mouse NIH 3T3 and 3) mouse SP2/0 lysate with Rad9b antibody. Expected molecular weight: 46-60 kDa.
Flow cytometry testing of mouse HEPA1-6 cells with Rad9b antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Rad9b antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Rad9b antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
By coimmunoprecipitation of erythroleukemia cells transfected with RAD9B, it was found that RAD9B associated with 3 endogenous checkpoint proteins, HUS1, RAD1, and RAD17, possibly in a 9-1-1-like complex. RAD9B also interacted with transfected HUS1B. It was also found decreased abundance of RAD9B mRNA in testicular seminomas relative to normal tissue controls and other types of testicular tumors.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids M1-G403) was used as the immunogen for the Rad9b antibody.
Limitation:
This Rad9b antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
Q6WBX7