Tigit Antibody / Vstm3 / Vsig9

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NSJ Bioreagents
Product Code: NSJ-RQ6213
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6213-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Tigit antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 3
IHC staining of FFPE mouse spleen with Tigit antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 3
Western blot testing of 1) rat spleen, 2) rat plasma and 3) mouse spleen lysate with Tigit antibody. Predicted molecular weight ~26 kDa.
3 / 3
Flow cytometry testing of mouse RAW264.7 cells with Tigit antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tigit antibody.
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IHC staining of FFPE mouse spleen with Tigit antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat spleen, 2) rat plasma and 3) mouse spleen lysate with Tigit antibody. Predicted molecular weight ~26 kDa.
Flow cytometry testing of mouse RAW264.7 cells with Tigit antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tigit antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Tigit antibody should be determined by the researcher.
Description:
TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is an immune receptor present on some T cells and Natural Killer Cells (NK). This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A mouse recombinant partial protein (amino acids T29-G249) was used as the immunogen for the Tigit antibody.
Limitation:
This Tigit antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P86176