Cd79a Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ5965
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ5965-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Cd79a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 6
Immunofluorescent staining of FFPE mouse intestinal lymph node cells with Cd79a antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
Immunofluorescent staining of FFPE rat intestine with Cd79a antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
3 / 6
IHC staining of FFPE mouse thymus with Cd79a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat thymus with Cd79a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of rat spleen lysate with Cd79a antibody. Expected molecular weight: 25-47 kDa depending on glycosylation level.
6 / 6
Flow cytometry testing of mouse spleen cells with Cd79a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd79a antibody.
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Immunofluorescent staining of FFPE mouse intestinal lymph node cells with Cd79a antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat intestine with Cd79a antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
IHC staining of FFPE mouse thymus with Cd79a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat thymus with Cd79a antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of rat spleen lysate with Cd79a antibody. Expected molecular weight: 25-47 kDa depending on glycosylation level.
Flow cytometry testing of mouse spleen cells with Cd79a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Cd79a antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry: 1-2ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Cd79a antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Cluster of differentiation CD79A also known as B-cell antigen receptor complex-associated protein alpha chain and MB-1 membrane glycoprotein, is a protein that in humans is encoded by the CD79A gene. It is mapped to 7 A3; 7 13.49 cM. The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant mouse protein (amino acids L29-P220) was used as the immunogen for the Cd79a antibody.
Limitation:
This Cd79a antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P11911