Tspan1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ6215
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6215-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the Tspan1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE rat stomach tissue with Tspan1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of rat small intestine with Tspan1 antibody. Expected molecular weight: 28-30 kDa.
3 / 3
Flow cytometry testing of rat RH35 cells with Tspan1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tspan1 antibody.
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Immunofluorescent staining of FFPE rat stomach tissue with Tspan1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of rat small intestine with Tspan1 antibody. Expected molecular weight: 28-30 kDa.
Flow cytometry testing of rat RH35 cells with Tspan1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Tspan1 antibody.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml,Immunofluorescence: 5ug/ml
Application Note:
Optimal dilution of the Tspan1 antibody should be determined by the researcher.
Description:
TSPAN1 belongs to the large family of tetraspanins, so named because they contain 4 transmembrane domains. Tetraspanins are frequently expressed at the cell surface in association with each other and with other molecules, such as integrins, and they function to regulate cell adhesion, migration, proliferation, and differentiation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A rat recombinant partial protein (amino acids A125-A212) was used as the immunogen for the Tspan1 antibody.
Limitation:
This Tspan1 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Rat
Uniprot #:
Q6AYR9