Il4r Antibody / Il4 receptor

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ6186
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ6186-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
After reconstitution, the Il4 receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Images

1 / 2
Flow cytometry testing of mouse spleen cells with Il4 receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL4 Receptor antibody.
2 / 2
IHC staining of FFPE rat spleen with Il4 receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
❮❯

Flow cytometry testing of mouse spleen cells with Il4 receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IL4 Receptor antibody.
IHC staining of FFPE rat spleen with Il4 receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Il4 receptor antibody should be determined by the researcher.
Description:
IL4R (INTERLEUKIN 4 RECEPTOR) is a human gene that encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. The IL4R gene is localized to 16p12.1-p11.2 by in situ hybridization and Southern blot analysis of DNA from a panel of mouse-human somatic cell lines. IL4 plays a major role in immunoglobulin E(IgE) production. Its signal is conferred to effector cells through binding to the alpha chain of the IL4 receptor. IL4 interacts with IL4R with high affinity, leading to dimerization with either the common gamma chain,a component of receptors for a number of cytokines, to create a type I receptor, or with IL13RA1 to form a type II receptor.The IL4R gene contains 12 exons and IL4R is a complex of at least 2 components, one of which is a novel affinity converting subunit that is critical for cellular signal transduction. See also IL13RA1 and IL13RA2.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A rat recombinant partial protein (amino acids I26-E719) was used as the immunogen for the Il4 receptor antibody.
Limitation:
This Il4 receptor antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Rat, Mouse
Uniprot #:
Q63257