Beta Tubulin Antibody Loading Control

NSJ Bioreagents
Product Code: NSJ-N1038
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-N1038-25UG25 ug£254.00
Quantity:
NSJ-N1038-100UG100 ug£450.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Non-Human Primates
  • Rat
Application: Western Blot (WB)
Storage:
Aliquot the loading control antibody and store frozen at -20oC or colder to avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human samples with beta Tubulin antibody at 2ug/ml. Predicted molecular weight: 50-55 kDa.~
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Western blot testing of mouse samples with beta Tubulin antibody at 2ug/ml. Predicted molecular weight: 50-55 kDa.

Western blot testing of human samples with beta Tubulin antibody at 2ug/ml. Predicted molecular weight: 50-55 kDa.~
Western blot testing of mouse samples with beta Tubulin antibody at 2ug/ml. Predicted molecular weight: 50-55 kDa.

Further Information

Application Details :
Western blot: 2-4ug/ml
Application Note:
Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
Buffer:
1X PBS, pH 7.4
Description:
There are five mammalian members of the tubulin family: alpha, beta, gamma, delta, and epsilon. Heterodimers of alpha and beta tubulin make up microtubules, which along with microfilaments and intermediate filaments, make up the cytoskeleton. Due to its ubiquitous presence in mammalian cells, it is often used a loading control protein in western blot. An antibody to beta-tubulin is used to demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Format :
Purified
Formulation :
0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Gene ID #:
203068
Immunogen:
A recombinant protein fragment from the C-terminal region of human beta tubulin was used as the immunogen for this antibody.
Limitation:
This beta Tubulin antibody loading control is available for research use only.
Purity:
Protein A Affinity Chromatography
Species Reactivity :
Human, Mouse, Rat, Primate, Dog