Chicken Cort(Corticosterone) ELISA Kit

ELK Biotechnology
Product Code: ELK8925
Product Group: ELISA Kits
CodeSizePrice
ELK8925-48T48T£261.00
Quantity:
ELK8925-96T96T£335.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Regulatory Status: RUO
Target Species: Chicken (Gallus)
Application: Enzyme-Linked Immunosorbent Assay (ELISA)

Video

Further Information

Alternative Names:
Corticosrone
Assay Length:
2h
Assay Type:
Competitive Inhibition
Detection Range:
1.57-100 ng/mL
Sample Type:
serum, plasma, urine and other biological fluids
Sensitivity:
0.51 ng/mL
Standard:
100 ng/mL
Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken Cort protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken Cort. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Chicken Cort in the samples is then determined by comparing the OD of the samples to the standard curve.